1:00 pm - 2:00 pm
318J Agriculture/Forestry Centre (AgFor), Agriculture/Forestry Centre, Edmonton AB
Event details: A graduate exam seminar is a presentation of the student’s final research project for their degree.
This is an ALES MSc Final Exam Seminar by Yeye Lu. This seminar is open to the general public to attend.
Thesis Topic: Fermentation of Air Currents Particle Separation (ACAPS) by-product streams for bioethanol production
Bioethanol has long been considered as one of the most promising renewable biofuels alternatives to fossil fuels. To meet the increasing demand for global bioethanol production, it is necessary to identify a cost-effective feedstock alternative. New feedstocks will help alleviate economic stress on ethanol production which currently utilizes conventional starchy or sugary materials, such as corn and wheat. One such promising alterative is barley starch concentrate, a by-product generated during isolation of β-glucan from barley grains via the Air Currents Assisted Particle Separation (ACAPS) technology. To investigate this alternative feedstock, the effect of omitting enzymes during hydrolysis of barley starch concentrate mash was studied. Results demonstrated that complete hydrolysis of barley starch concentrate mash can be achieved with only STARGENTM 002, not requiring FERMGENTM 2.5X, OPTIMASHTM TBG and GC 626, which are typically required for hydrolysis of wheat. Results also showed that hydrolysis of barley starch concentrate mash with a 0.5X dosage of STARGENTM 002 showed similar enzymatic kinetics to that of the wheat benchmark using all four enzymes. Furthermore, the fermentation of barley starch concentrate demonstrated that fermentations incorporating barley starch concentrate and a 0.25X dosage of STARGENTM 002 (with no FERMGENTM, OPTIMASHTM TBG and GC 626) displayed similar ethanol efficiency as the wheat benchmark, however, they required phosphorus supplementation. The results of this study demonstrate that barley starch concentrate is a promising feedstock for bioethanol production as compared to the wheat benchmarks commonly used in industry. This study successfully established the simultaneous saccharification and fermentation (SSF) while controlling rates of hydrolysis and avoiding osmotic stress on yeasts. As a result, considerable cost savings are possible during production of bioethanol from barley starch concentrate through the use of decreased dosages of STARGENTM 002, and the omission of all other enzymes typically used in wheat fermentations.
MSc with Dr. David Bressler