Momna Farzand | ALES Graduate Seminar

Date(s) - 31/08/2021
12:00 pm - 1:00 pm

A graduate exam seminar is a presentation of the student’s final research project for their degree.
This is an ALES MSc Final Exam Seminar by Momna Farzand. This seminar is open to the general public to attend.

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Meeting ID: 986 7786 2934
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Meeting ID: 986 7786 2934
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Thesis Topic: Genetic Mapping for Stripe Rust Resistance in Canadian Spring Wheat Mapping Populations

MSc with Dr. Dean Spaner.

Seminar Abstract:

Stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Erikss. (Pst), is one of the most devastating diseases of wheat (Triticum aestivum L.) globally. Exploring and utilising new sources of resistance is essential for breeding resistant wheat cultivars. The objective of this thesis was to identify QTL associated with stripe rust resistance in two hexaploid spring wheat populations. In the first study, a doubled haploid (DH) population (n=291) derived from the cross AAC Innova/AAC Proclaim was evaluated to dissect the genetics of resistance in cv. AAC Innova. This population was evaluated for stripe rust severity in disease nurseries at Creston, British Columbia (in 2016 and 2020) and Lethbridge, Alberta (in 2016, 2017 and 2020), and genotyped using the wheat 90K SNP (single nucleotide polymorphism) assay. A high-density genetic map was constructed consisting of 7,112 SNP markers with an average marker interval of 2.3 cM. Quantitative trait loci (QTL) mapping identified one major (QYr.lrdc-2A) and ten minor effect (QYr.lrdc-2B.1, QYr.lrdc-2B.2, QYr.lrdc-2B.3, QYr.lrdc-2B.4, QYr.lrdc-2D, QYr.lrdc-3B, QYr.lrdc-5A, QYr.lrdc-5B, QYr.lrdc-5D and QYr.lrdc-7D) loci. In the second study, a recombinant inbred line (RIL) population of 252 individuals was developed from the cross AAC Cameron/P2711. This population was evaluated for stripe rust severity at the adult plant stage at the nurseries in Creston, BC (in 2018, 2019 and 2020) and Lethbridge, AB (in 2018 and 2020), and was genotyped using the wheat 90K Infinium iSelect SNP assay. A high-density genetic map of 8,914 SNP markers was constructed covering all the wheat chromosomes. Four resistance alleles were contributed by the male parent P2711 and three from the female parent AAC Cameron. The stable QTLs and their closely associated markers identified in these studies could be utilized in marker assisted selection for stripe rust resistant cultivar development.