Date(s) - 14/12/2021
1:00 pm - 2:00 pm
Event details: A graduate exam seminar is a presentation of the student’s final research project for their degree.
This is an ALES MSc Final Exam Seminar by Izabela Ciechanowska . This seminar is open to the general public to attend.
Quantitative trait loci mapping of rust resistance and agronomic traits in the doubled haploid spring wheat population ‘HYAYT12-10’ x ‘GP146’
MSc with Dr. Dean Spaner
Marker-assisted selection requires the identification of molecular markers associated with major genes and quantitative trait loci (QTL) using linkage analysis. In this study, we used 167 doubled haploid (DH) lines derived from two unregistered spring wheat (Triticum aestivum L.) parental lines that belong to the Canada Western Special Purpose (CWSP) class to map QTLs associated with five traits using inclusive composite interval mapping (ICIM). Using ICIM, least square means phenotype data across 3-4 environments, and genetic map of 2,676 SNPs out of the wheat 90K SNP array, we identified ten QTLs associated with maturity (4A and 5B), plant lodging (4B, 5A, 5D, and 7D), grain yield (2D), leaf rust (4A) and stem rust (1A and 2B). Each QTL individually accounted for 6.0-22.3% of the phenotypic variance and together accounted for 8.6-38.2% of each trait. QTLs identified for rusts using ICIM had a minor effect (6.0-9.0%) or a major effect (22.3%). Our major effect QTL at 22.3% was discovered on chromosome 2B and contributed to stem rust response. Its physical location has been associated with disease response in previous studies. Results from this study provide additional valuable information to wheat researchers, in particular the area on chromosome 2B should be considered for future analyses.